|
KMID : 0363420180320020001
|
|
Journal of Korean Oriental Pediatrics
2018 Volume.32 No. 2 p.1 ~ p.10
|
|
|
Effects of Douchi Hataedock Treatment on Induction of Allergic Rhinitis in Obese Induced NC/Nga Mice
|
|
Ahn Sang-Hyun
Kim Ki-Bong
|
|
|
Abstract
|
|
|
Objectives: This study investigated the effects of Hataedock treatment with Douchi on induction of allergic rhinitis in obese induced NC/Nga mice.
Methods: NC/Nga mice were divided into control group (Ctrl), allergic rhinitis induced obese mice group (ARE), and allergic rhinitis induced obese mice group with Douchi Hataedock treatment (FGT). The 3-week-old mice of the FGT group were given one 10 mg/kg dose of Douchi Hataedock extract and sensitized with allergic antigens at weeks 4, 5, and 6. After 1 week of final sensitization, allergic rhinitis was induced primarily in mice nasal cavities for five days. After one week of the completion with the first induction, the second induction was introduced by the same method. After 1 week, few samples of the nasal mucosal tissues of each group were prepared. The factor of Th2 differentiation and inflammation control such that IL-4, STAT6, CD40, FcεRI, substance P, MMP-9, NF-κB p65, p-IkB, iNOS and COX-2 were observed by immunohistochemistry. Also, the difference in nasal mucosal injury was observed by histochemical method (PAS staining).
Results: The FGT group showed that reduced IL-4 production, STAT6 expression and CD40 expression by regulating excessive Th2 differentiation. Also, production of substance P and MMP-9 and activity of FcεRI in mast cells were decreased. Inhibition of NF-kB p65 activity was induced by inhibition of p-IkB, and the production of inflammatory enzymes iNOS and COX-2 were decreased. In addition, the damage of intramural respiratory epithelium was low and excessive mucin secretion in goblet cells was low.
Conclusions: This study confirmed the possibility of controlling the allergic rhinitis in obese children who are expected to have an overactive inflammation.
|
|
|
KEYWORD
|
|
|
Hataedock , Douchi , Obesity , Allergic rhinitis , Th2 differentiation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|